Haplomitrium blumii (Nees) R. M. Schust. Axenic culture on agar maintained in an environmental chamber.

Our axenic cultures are initiated from mature capsules under sterilize conditions as shown below with graduate student Annie Peng at our laminar flow hood. We use Hatcher's culture media (Bryologist 68: 227-231. 1965) in recycled baby-food jars with special caps purchased from Sigma Scientific.



pic

Protocol for preparing nutrient agar

Protocol for establishing and maintaining axenic cultures

Bryophytes in culture in the Crandall-Stotler laboratory. (March, 2005).